Methods in Proteome and Protein Analysis

✍ Scribed by Mar Orzáez, Francisco J. Taberner (auth.), Professor Dr. Roza Maria Kamp, Dr. Juan J. Calvete, Ass. Prof. Dr. Theodora Choli-Papadopoulou (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2004
• Tongue: English
• Leaves: 428
• Series: Principles and Practice
• Edition: 1
• Category: Library

✦ Synopsis

Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14^th MPSA meeting.

Selected contributions discuss the latest techniques and approaches for analysing protein and proteome structures. Major topics include:

protein and peptide sample preparation and separation

new reagent for protein sequence analysis

mass spectrometry in protein research

analysis of posttranslational modification

protein-protein interaction using MALDI-MS

manipulation of genome or functional compositon trap

structure-function correlation study using optical biosensors of microcolorimetrical techniques

structural proteomics as NMR or fluorescence polarization study

the classification and prediction of structure or functional sites

in silico analysis of proteins and proteomes

increasing throughput and data quality for proteomics

✦ Table of Contents

Front Matter....Pages I-XXXII
Helix-Helix Packing Between Transmembrane Fragments....Pages 1-14
Mobility Studies in Proteins by 15 N Nuclear Magnetic Resonance: Rusticyanin as an Example....Pages 15-33
Structure and Dynamics of Proteins in Crowded Media: A Time-Resolved Fluorescence Polarization Study....Pages 35-48
Analyses of Wheat Seed Proteome: Exploring Protein-Protein Interactions by Manipulating Genome Composition....Pages 49-66
Modification-Specific Proteomic Strategy for Identification of Glycosyl-Phosphatidylinositol Anchored Membrane Proteins....Pages 67-79
Diocleinae Lectins: Clues to Delineate Structure/Function Correlations....Pages 81-91
The Contribution of Optical Biosensors to the Analysis of Structure-Function Relationships in Proteins....Pages 93-101
The Use of Protein—protein Interaction Networks for Genome Wide Protein Function Comparisons and Predictions....Pages 103-124
Probing Ribosomal Proteins Capable of Interacting with Polyamines....Pages 125-132
Applications of Optical Biosensors to Structure-Function Studies on the EGF/EGF Receptor System....Pages 133-164
The Functional Interaction Trap: A Novel Strategy to Study Specific Protein-Protein Interactions....Pages 165-182
Analysis of Protein—Protein Interactions in Complex Biological Samples by MALDI TOF MS. Feasibility and Use of the Intensity-Fading (IF-) Approach....Pages 183-202
Accelerator Mass Spectrometry in Protein Analysis....Pages 203-216
The Use of Microcalorimetric Techniques to Study the Structure and Function of the Transferrin Receptor from Neisseria meningitidis ....Pages 217-230
The Quantitative Advantages of an Internal Standard in Multiplexing 2D Electrophoresis....Pages 231-250
Genetic Engineering of Bacterial and Eukaryotic Ribosomal Proteins for Investigation on Elongation Arrest of Nascent Polypeptides and Cell Differentiation....Pages 251-259
MALDI-MS Analysis of Peptides Modified with Photolabile Arylazido Groups....Pages 261-268
A New Edman-Type Reagent for High Sensitive Protein Sequencing....Pages 269-278
Amino Acid Sequencing of Sulfonic Acid-Labeled Tryptic Peptides Using Post-Source Decay and Quadratic Field MALDI-ToF Mass Spectrometry....Pages 279-297
Separation of Peptides and Amino Acids using High Performance Capillary Electrophoresis....Pages 299-306
InterPro and Proteome Analysis — In silico Analysis of Proteins and Proteomes....Pages 307-318
Prediction of Functional Sites in Proteins by Evolutionary Methods....Pages 319-340
Extracting and Searching for Structural Information: A Multiresolution Approach....Pages 341-357
Peak Erazor: A Windows-Based Program for Improving Peptide Mass Searches....Pages 359-370
Increasing Throughput and Data Quality for Proteomics....Pages 371-397
Back Matter....Pages 399-404

✦ Subjects

Proteomics; Biochemistry, general; Bioinformatics