Proteome and Protein Analysis

✍ Scribed by D. R. Goodlett, A. Timperman, S. P. Gygi, J. Watts, G. Corthals, D. Figeys (auth.), Prof. Dr. Roza Maria Kamp, Prof. Dr. Dimitris Kyriakidis, Ass. Prof. Dr. Theodora Choli-Papadopoulou (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2000
• Tongue: English
• Leaves: 370
• Edition: 1
• Category: Library

✦ Synopsis

Selected papers presented at the MPSA 98 are covering new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the total cell proteins, the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed.
Topics included are: Protein sequencing analysis, protein and peptide sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and protein folding, functional implications of protein domains and newly emerging methods for the investigation of the proteome, allowing to analyse the expression of genes

✦ Table of Contents

Front Matter....Pages I-XI
Front Matter....Pages 1-1
Strategies and Methods for Proteome Analysis....Pages 3-14
Protein Sequencing or Genome Sequencing. Where Does Mass Spectrometry Fit into the Picture?....Pages 15-27
Reversible Negative Staining of Protein on Electrophoresis Gels by Imidazole-Zinc Salts: Micropreparative Applications to Proteome Analysis by Mass Spectrometry....Pages 29-52
Direct Analysis of Protein Complexes....Pages 53-64
De novo Sequencing of Proteins With Mass Spectrometry Using the Differential Scanning Technique....Pages 65-79
Characterization of Gel Separated Proteins....Pages 81-87
Front Matter....Pages 89-90
Protein Structure and Dynamics by NMR in Solution....Pages 91-116
Elucidation of Functionally Significant Structural Modifications by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry with Post-Source Decay Analysis....Pages 117-138
Detection of Specific Zinc Finger Peptide Complexes with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry....Pages 139-147
Epitope Mapping for the Monoclonal Antibody that Inhibits Intramolecular Flavin to Heme Electron Transfer in Flavocytochrome b 2 from Baker’s Yeast (L-Lactate Dehydrogenase)....Pages 149-155
A MALDI-TOF Mass Spectrometry Approach to Investigate the Defense Reactions in Drosophila melanogaster , an Insect Model for the Study of Innate Immunity....Pages 157-174
A New Method for the Isolation of the C-Terminal Peptide of Proteins by the Combination of Selective Blocking of Proteolytic Peptides and Cation Exchange Chromatography....Pages 175-187
The Folding Pathway of Disulfide Containing Proteins....Pages 189-197
Cavity Supported HPLC of Cis/Trans Isomers of Proline Containing Peptides Using Cyclodextrins and Calixarenes....Pages 199-210
The Perception of Hydrophobic Clusters in the Native and Partially Unfolded States of a Protein....Pages 211-224
Structure, Dynamics and Function of the Proton Pump Bacteriorhodopsin....Pages 225-240
Structural Characterisation of Porcine Seminal Plasma Psp-I/Psp-II, a Paradigm Spermadhesin Molecule Built by Heterodimerization of Glycosylated Subunits....Pages 241-250
Expression and Characterization of Saposin-Like Proteins....Pages 251-254
Front Matter....Pages 255-255
A Structural Model for the P53 Complex with DNA Response Elements: Implications for P53 Function and Future Research Directions....Pages 257-272
Overproduction, Purification and Structural Studies on the Zn Containing S14 Ribosomal Protein from Thermus thermophilus ....Pages 273-288
Front Matter....Pages 255-255
Biomolecular Interaction of Matrix Metalloproteinases and Their Inhibitors TIMPs....Pages 289-300
Front Matter....Pages 301-301
Enzymatic and Chemical Deblocking of N-Terminally Modified Proteins....Pages 303-319
Exploring Functions for Glycosylation in Host Defence using Novel Oligosaccharide Sequencing Technology....Pages 321-339
Proteoglycans: Biological Roles and Strategies for Isolation and Determination of Their Glycan Constituents....Pages 341-363
Back Matter....Pages 365-372

✦ Subjects

Biochemistry, general; Cell Biology