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Proteomic Analysis of Trypanosoma cruzi Developmental Stages Using Isotope-Coded Affinity Tag Reagents

โœ Scribed by Paba, Jaime; Ricart, Carlos A. O.; Fontes, Wagner; Santana, Jaime M.; Teixeira, Antonio R. L.; Marchese, Jason; Williamson, Brian; Hunt, Tony; Karger, Barry L.; Sousa, Marcelo V.

Book ID
121350633
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
169 KB
Volume
3
Category
Article
ISSN
1535-3893

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The combination of isotope coded affinity tag (ICAT) reagents and tandem mass spectrometry constitutes a new method for quantitative proteomics. It involves the site-specific, covalent labeling of proteins with isotopically normal or heavy ICAT reagents, proteolysis of the combined, labeled protein