Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
β Scribed by Gygi, Steven P.; Rist, Beate; Gerber, Scott A.; Turecek, Frantisek; Gelb, Michael H.; Aebersold, Ruedi
- Book ID
- 109513261
- Publisher
- Nature Publishing Group
- Year
- 1999
- Tongue
- English
- Weight
- 495 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/13690
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## Abstract Affinity captureβrelease electrospray ionization mass spectrometry (ACESIMS) and isotopeβcoded affinity tags (ICAT) are two recently introduced techniques for the quantitation of protein activity and content with applications to clinical enzymology and functional proteomics, respectivel
The combination of isotope coded affinity tag (ICAT) reagents and tandem mass spectrometry constitutes a new method for quantitative proteomics. It involves the site-specific, covalent labeling of proteins with isotopically normal or heavy ICAT reagents, proteolysis of the combined, labeled protein