Optimization of the Isotope-Coded Affini
Optimization of the Isotope-Coded Affinity Tag-Labeling Procedure for Quantitative Proteome Analysis
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Marcus B. Smolka; Huilin Zhou; Subhasish Purkayastha; Ruedi Aebersold
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Article
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2001
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Elsevier Science
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English
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The combination of isotope coded affinity tag (ICAT) reagents and tandem mass spectrometry constitutes a new method for quantitative proteomics. It involves the site-specific, covalent labeling of proteins with isotopically normal or heavy ICAT reagents, proteolysis of the combined, labeled protein