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Protein Signal Assignments Using Specific Labeling and Cell-Free Synthesis

✍ Scribed by Jianxia Shi; Jeffrey G. Pelton; Ho S. Cho; David E. Wemmer


Book ID
111591350
Publisher
Springer Netherlands
Year
2004
Tongue
English
Weight
382 KB
Volume
28
Category
Article
ISSN
0925-2738

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Cell-free synthesis and amino acid-selec
✍ Takanori Kigawa; Yutaka Muto; Shigeyuki Yokoyama πŸ“‚ Article πŸ“… 1995 πŸ› Springer Netherlands 🌐 English βš– 536 KB

For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system