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High level cell-free expression and specific labeling of integral membrane proteins

✍ Scribed by Christian Klammt; Frank Löhr; Birgit Schäfer; Winfried Haase; Volker Dötsch; Heinz Rüterjans; Clemens Glaubitz; Frank Bernhard

Book ID
110914894
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
443 KB
Volume
271
Category
Article
ISSN
1432-1327

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High-level cell-free synthesis yields of
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✍ Aaron R. Goerke; James R. Swartz 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 500 KB 👁 2 views

## Abstract We describe an __E. coli__‐based cell‐free system for the production of proteins with a non‐natural amino acid (nnAA) incorporated site‐specifically (modified protein). The mutant __Methanococcus jannaschii__ tyrosyl‐tRNA synthetase (mTyrRS) and tRNA^Tyr^ pair were used as orthogonal el