## Abstract The use of cellβfree protein synthesis (CFPS) for recombinant protein production is emerging as an important technology. For example, the openness of the cellβfree system allows control of the reaction environment to promote folding of disulfide bonded proteins in a rapid and economical
Microbeads display of proteins using emulsion PCR and cell-free protein synthesis
β Scribed by Rui Gan; Yumiko Yamanaka; Takaaki Kojima; Hideo Nakano
- Publisher
- American Institute of Chemical Engineers
- Year
- 2008
- Tongue
- English
- Weight
- 328 KB
- Volume
- 24
- Category
- Article
- ISSN
- 8756-7938
- DOI
- 10.1002/btpr.43
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## Abstract Herein we describe the methods for selective and reversible regulation of gene expression using antisense oligodeoxynucleotides (ODNs) in a cellβfree protein synthesis system programmed with multiple DNAs. Either a complete shut down or controlled level of gene expression was attained t
A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from