Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

• Year: 2011
• Tongue: English
• Leaves: 521
• Series: Methods of Biochemical Analysis
• Category: Library

✦ Synopsis

This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:

• Gel filtration and ion exchange chromatography
• Affinity and covalent chromatography 
• Reversed phase and hydrophobic interaction chromatography 
• Ligands from combinatorial libraries 
• Electrophoresis in gels 
• Immunoelectrophoresis and capillary electrophoresis 
• Two-dimensional electrophoresis in proteomics
• Protein elution and blotting techniques
• Large-scale electrophoresis processes

Content:
Chapter 1 Introduction to Protein Purification (pages 1–22): Bo Ersson, Lars Ryden and Jan?Christer Janson
Chapter 2 Introduction to Chromatography (pages 23–50): Jan?Christer Janson and Jan Ake Jonsson
Chapter 3 Gel Filtration: Size Exclusion Chromatography (pages 51–91): Lars Hagel
Chapter 4 Ion Exchange Chromatography (pages 93–133): Evert Karlsson and Irwin Hirsh
Chapter 5 High?Resolution Reversed?Phase Chromatography of Proteins (pages 135–164): Sylvia Winkel Pettersson
Chapter 6 Hydrophobic Interaction Chromatography (pages 165–181): Kjell?Ove Eriksson and Makonnen Belew
Chapter 7 Immobilized Metal Ion Affinity Chromatography (pages 183–201): Lennart Kagedal
Chapter 8 Covalent Chromatography (pages 203–219): Francisco Batista?Viera, Lars Ryden and Jan Carlsson
Chapter 9 Affinity Chromatography (pages 221–258): Francisco Batista?Viera, Jan?Christer Janson and Jan Carlsson
Chapter 10 Affinity Ligands from Chemical Combinatorial Libraries (pages 259–267): Enrique Carredano and Herbert Baumann
Chapter 11 Affinity Ligands from Biological Combinatorial Libraries (pages 269–278): Per?Ake Nygren
Chapter 12 Membrane Separations (pages 279–317): Joachim K. Walter, Zuwei Jin, Maik W. Jornitz and Uwe Gottschalk
Chapter 13 Refolding of Inclusion Body Proteins from E. coli (pages 319–338): Zhiguo Su, Diannan Lu and Zheng Liu
Chapter 14 Purification of PEGylated Proteins (pages 339–362): Conan J. Fee and James M. Van Alstine
Chapter 15 Electrophoresis in Gels (pages 363–377): Reiner Westermeier
Chapter 16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients (pages 379–409): Pier Giorgio Righetti, Elisa Fasoli and Sabina Carla Righetti
Chapter 17 Two?Dimensional Electrophoresis in Proteomics (pages 411–439): Reiner Westermeier and Angelika Gorg
Chapter 18 Protein Elution and Blotting Techniques (pages 441–450): Reiner Westermeier
Chapter 19 Capillary Electrophoretic Separations (pages 451–485): Wolfgang Thormann
Chapter 20 High Throughput Screening Techniques in Protein Purification (pages 487–506): Karol M. Lacki and Eggert Brekkan