This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organi
Protein Purification: Principles, High Resolution Methods, and Applications
β Scribed by Jan-Christer Janson
- Publisher
- Wiley
- Year
- 2011
- Tongue
- English
- Leaves
- 522
- Edition
- 3
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
The authoritative guide on protein purificationβnow completely updated and revised
Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.
Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:
High-resolution reversed-phase liquid chromatography
Electrophoresis in gels
Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients
Affinity ligands from chemical and biological combinatorial libraries
Membrane separations
Refolding of inclusion body proteins from E. coli
Purification of PEGylated proteins
High throughput screening techniques in protein purification
The history of protein chromatography
π SIMILAR VOLUMES
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organi
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organi
<p><P>Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In <EM>High Throughput Protein Expression and Purification: Methods and Protocols</EM>, leading scientists detail the most successful protocols
<p><P>Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In <EM>High Throughput Protein Expression and Purification: Methods and Protocols</EM>, leading scientists detail the most successful protocols