Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis)

✍ Scribed by Jan-Christer Janson

• Publisher: Wiley
• Year: 2011
• Tongue: English
• Leaves: 522
• Series: Methods of Biochemical Analysis
• Edition: 3
• Category: Library

✦ Synopsis

This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography  Reversed phase and hydrophobic interaction chromatography  Ligands from combinatorial libraries  Electrophoresis in gels  Immunoelectrophoresis and capillary electrophoresis  Two-dimensional electrophoresis in proteomics Protein elution and blotting techniques Large-scale electrophoresis processes

✦ Table of Contents

Front Cover......Page 1
Half-title......Page 2
Inside Cover......Page 3
Copyright......Page 4
Contents......Page 5
Preface to the 3rd Edition......Page 7
Preface to the 2nd Edition......Page 9
Preface to the 1st Edition......Page 10
Contributors......Page 12
Color Plates......Page 14
Part I - Introduction......Page 22
1: Introduction to Protein Purification......Page 23
Part II - Chromatography......Page 43
2: Introduction to Chromatography......Page 44
3: Gel Filtration: Size Exclusion Chromatography......Page 70
4: Ion Exchange Chromatography......Page 111
5: High-Resolution Reversed-Phase Chromatography of Proteins......Page 152
6: Hydrophobic Interaction Chromatography......Page 182
7: Immobilized Metal Ion Affinity Chromatography......Page 199
8: Covalent Chromatography......Page 218
9: Affinity Chromatography......Page 235
10: Affinity Ligands From Chemical Combinatorial Libraries......Page 273
11: Affinity Ligands From Biological Combinatorial Libraries......Page 282
Part III - Other Separation Methods and Related Techniques......Page 292
12: Membrane Separations......Page 293
13: Refolding of Inclusion Body Proteins From E. coli......Page 330
14: Purification of Pegylated Proteins......Page 350
Part IV - Electrophoresis......Page 374
15: Electrophoresis in Gels......Page 375
16: Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients......Page 388
17: Two-Dimensional Electrophoresis in Proteomics......Page 419
18: Protein Elution and Blotting Techniques......Page 448
19: Capillary Electrophoretic Separations......Page 458
Part V - Separation Method Optimization......Page 493
20: High Throughput Screening Techniques in Protein Purification......Page 494
index......Page 512