𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Protein folding in the periplasm of Escherichia coli

✍ Scribed by Christoph Wülfing; Andreas Plückthun


Book ID
110093391
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
776 KB
Volume
12
Category
Article
ISSN
0950-382X

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Expression of correctly folded proteins
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Many heterologous polypeptides fail to fold into their native state when expressed in Escherichia co~i; instead, they are either degraded by the cellular proteolytic machinery or accumulate in insoluble form, typically as inclusion bodies. Misfolding is a particularly vexing problem in the expressio

Folding and assembly of oligomeric prote
✍ Carolyn M. Teschke; Jonathan King 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 640 KB

High levels of expression of oligomeric proteins in heterologous systems are frequently associated with misfolding and accumulation of the polypeptides in inclusion bodies. This reflects aspects of the folding and assembly pathways of oligomeric proteins, which generally proceed from either folding