✦ LIBER ✦

Folding and assembly of oligomeric proteins in Escherichia coli

✍ Scribed by Carolyn M. Teschke; Jonathan King

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 640 KB
Volume: 3
Category: Article
ISSN: 0958-1669
DOI: 10.1016/0958-1669(92)90073-r

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✦ Synopsis

High levels of expression of oligomeric proteins in heterologous systems are frequently associated with misfolding and accumulation of the polypeptides in inclusion bodies. This reflects aspects of the folding and assembly pathways of oligomeric proteins, which generally proceed from either folding intermediates or native-like metastable species that are not in their final conformation. Methods for optimizing the yield of correctly assembled oligomers are discussed.

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