RNase H 0 reverse transcriptase (GIBCO-BRL, Gaithersburg, MD). ase chain reaction; SSCP, single-strand conformation polymorphism; mRNA, messenger p21 WAF1/CIP1 messenger RNA (mRNA) was amplified with the follow-RNA; cdk, cyclin dependent kinase.
Protein expression of p53, p21 (WAF1/CIP1), bcl-2, Bax, cyclin D1 and pRb in human colon carcinomas
β Scribed by I. K. Bukholm; J. M. Nesland
- Publisher
- Springer
- Year
- 2000
- Tongue
- English
- Weight
- 157 KB
- Volume
- 436
- Category
- Article
- ISSN
- 1432-2307
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p21 WAF1/Cip1 is an inhibitor of cdk/cyclin complexes, and thus regulates the cell cycle. p21 is also related to cell differentiation and is regulated by wild-type p53, although p53-independent regulatory pathways have been proposed. In order to analyse p21 expression as well as its relationship wit
The expression and prognostic role of cyclin D1, cyclin E, and p21 (WAF1/CIP1) were immunohistochemically investivated in 413 curatively resected gastric carcinomas. p21 was expressed in 65β’4 per cent (n=270), cyclin D1 in 23β’7 per cent (n=98), and cyclin E in 13β’6 per cent (n=56) of the tumours. Th