The cyclin-dependent kinase inhibitor p21/WAF1 is regulated by p53-dependent and p53-independent pathways. In addition, p21/WAF1 binds with proliferating cell nuclear antigen (PCNA) and inhibits the action of PCNA. To investigate the possible role of p21/WAF1 in human hepatocellular carcinomas (HCCs
Reduced p21WAF1/CIP1 expression and p53 mutation in hepatocellular carcinomas
β Scribed by A M Hui; Y Kanai; M Sakamoto; H Tsuda; S Hirohashi
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 352 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0270-9139
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β¦ Synopsis
RNase H 0 reverse transcriptase (GIBCO-BRL, Gaithersburg, MD). ase chain reaction; SSCP, single-strand conformation polymorphism; mRNA, messenger p21 WAF1/CIP1 messenger RNA (mRNA) was amplified with the follow-RNA; cdk, cyclin dependent kinase.
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Data are non-existent regarding coincidental alterations in the expression of p53 and its downstream target genes MDM2 and p21 Waf1/Cip1 in gastric carcinogenesis. An immunohistochemical study was therefore performed to examine the interrelationships of p53, MDM2, and p21 Waf1/Cip1 expression in a s