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Protein encapsulation in polyelectrolyte microcapsules for antigen delivery

โœ Scribed by M.L. De Temmerman; F. De Vos; J. Demeester; S.C. De Smedt

Book ID
113693442
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
173 KB
Volume
148
Category
Article
ISSN
0168-3659

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To assess processing of antigen-loaded microcapsules, DCs were incubated with OVA-loaded microcapsules and fluorescence intensity in the green channel (FL1-H) was measured by flow cytometry. Cells were gated first on the basis of a high side and forward scatter profile. Green autofluorescence of DCs