Polyelectrolyte Microcapsules as Antigen Delivery Vehicles To Dendritic Cells: Uptake, Processing, and Cross-Presentation of Encapsulated Antigens

✍ Scribed by Stefaan De Koker; Bruno G. De Geest; Satwinder K. Singh; Riet De Rycke; Thomas Naessens; Yvette Van Kooyk; Jo Demeester; Stefaan C. De Smedt; Johan Grooten

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 713 KB
• Volume: 121
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/ange.200903769

✦ Synopsis

To assess processing of antigen-loaded microcapsules, DCs were incubated with OVA-loaded microcapsules and fluorescence intensity in the green channel (FL1-H) was measured by flow cytometry. Cells were gated first on the basis of a high side and forward scatter profile. Green autofluorescence of DCs and green fluorescence emitted by the OVA-DQ microcapsule suspension were measured separately.