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Polyelectrolyte Microcapsules as Antigen Delivery Vehicles To Dendritic Cells: Uptake, Processing, and Cross-Presentation of Encapsulated Antigens

✍ Scribed by Stefaan De Koker; Bruno G. De Geest; Satwinder K. Singh; Riet De Rycke; Thomas Naessens; Yvette Van Kooyk; Jo Demeester; Stefaan C. De Smedt; Johan Grooten

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
714 KB
Volume
48
Category
Article
ISSN
0044-8249

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Polyelectrolyte Microcapsules as Antigen
Polyelectrolyte Microcapsules as Antigen Delivery Vehicles To Dendritic Cells: Uptake, Processing, and Cross-Presentation of Encapsulated Antigens
✍ Stefaan De Koker; Bruno G. De Geest; Satwinder K. Singh; Riet De Rycke; Thomas N 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 713 KB

To assess processing of antigen-loaded microcapsules, DCs were incubated with OVA-loaded microcapsules and fluorescence intensity in the green channel (FL1-H) was measured by flow cytometry. Cells were gated first on the basis of a high side and forward scatter profile. Green autofluorescence of DCs