## Abstract The generation of free radicals and the resulting oxidative modification of cell structures are omnipresent in mammalian cells. This includes the permanent oxidation of proteins leading to the disruption of the protein structure and an impaired functionality. In consequence, these oxidi
β¦ LIBER β¦
Protein Degradation by the Proteasome and Its Implications in Aging
β Scribed by BERTRAND FRIGUET; ANNE-LAURE BULTEAU; NIKI CHONDROGIANNI; MARIANGELA CONCONI; ISABELLE PETROPOULOS
- Book ID
- 111396512
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 260 KB
- Volume
- 908
- Category
- Article
- ISSN
- 0890-6564
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## Abstract This article describes an undergraduate biochemistry laboratory investigating the ubiquitinβproteasome pathway in yeast. In this exercise, the enzyme Ξ²βgalactosidase (Ξ²βgal) is expressed in yeast under the control of a stress response promoter. Following exposure to heat stress to induc