Protein Backbone 1 H N − 13 C α and 15 N− 13 C α Residual Dipolar and J Couplings: New Constraints for NMR Structure Determination
✍ Scribed by Ding, Keyang; Gronenborn, Angela M.
- Book ID
- 126009093
- Publisher
- American Chemical Society
- Year
- 2004
- Tongue
- English
- Weight
- 53 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0002-7863
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📜 SIMILAR VOLUMES
Four simple and sensitive HNCO-based methods for measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) coupling constants in protein main chains are presented. Three of these experiments enable the simultaneous measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) couplings. Exploitation of th
Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN, H~) and 3J(HN,C') coupling constants in uniformly 13C-and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method