Protein assay in the microgram range: Modifications of the hiraoka-glick method
β Scribed by Maria L. Bade
- Book ID
- 107711474
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 488 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0003-2697
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A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.