Protein analysis as a simple method for the quantitative assessment of sewage sludge disintegration
β Scribed by Ulrich Schmitz; Christian R. Berger; Hermann Orth
- Book ID
- 117241679
- Publisher
- IWA Publishing
- Year
- 2000
- Tongue
- English
- Weight
- 259 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0043-1354
No coin nor oath required. For personal study only.
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Non-aqueous solutions of Ellman's reagent [5,5%-dithio(2-nitrobenzoic acid), DTNB] can be used to quantify thiols supported on macroporous polystyrene and TentaGel resins. Organic solutions of Ellman's reagent may also be used as a qualitative tests for thiols on a wider range of solid supports.
A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien