Protein adsorption onto ceramic surfaces

✍ Scribed by Takami, Yoshiyuki ;Yamane, Shingo ;Makinouchi, Kenzo ;Otsuka, Goro ;Glueck, Julie ;Benkowski, Robert ;Nos�, Yukihiko

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 488 KB
• Volume: 40
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199804)40:1<24::aid-jbm3>3.0.co;2-t

✦ Synopsis

Ceramics seldom have been used as bloodcontacting materials. However, alumina ceramic (Al 2 O 3 ) and polyethylene are incorporated into the pivot bearings of the Gyro centrifugal blood pump. This material combination was chosen based on the high durability of the materials. Due to the stagnant flow that often occurs in a continuous flow condition inside a centrifugal pump, pivot bearing system is extremely critical. To evaluate the thombogenicity of pivot bearings in the Gyro pump, this study sought to investigate protein adsorption, particularly albumin, IgG, fibrinogen, and fibronectin onto ceramic surfaces. Al 2 O 3 and silicon carbide ceramic (SiC) were compared with polyethylene (PE) and polyvinylchloride (PVC). Bicinchoninic acid (BCA) protein assay revealed that the amount of adsorbed proteins onto Al 2 O 3 and SiC was significantly less than that on PVC. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that numerous proteins adsorbed onto PVC compared to PE, Al 2 O 3 , and SiC. Identification of adsorbed proteins by Western immunoblotting revealed that the adsorption of albumin was similar on all four materials tested. Western immunoblotting also indicated lesser amounts of IgG, fibrinogen, and fibronectin on Al 2 O 3 and SiC than on PE and PVC. In conclusion, ceramics (Al 2 O 3 and SiC) are expected to be thromboresistant from the viewpoint of protein adsorption.