✦ LIBER ✦

Properties of an SV40-transformed hamster cell line resistant to actinomycin D

✍ Scribed by R. Wicker; M.-F. Bourali; H. G. Suarez; R. Cassingena

Publisher: John Wiley and Sons
Year: 1972
Tongue: French
Weight: 600 KB
Volume: 10
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910100323

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The biological properties of a hamster cell line Cl 2 TSV‐5/R derived from an SV40 tumour and made resistant to Actinomycin D (2 μg/ml) were investigated, in particular: tumorigenicity in vitro growth on plastic or in soft agar, agar, persistence of SV40 specific neoantigens (T and TSTA), and caryotype. When compared to the parental cell line (Cl 2 TSV‐5), the tumorigenicity and the ability to grow in soft agar of the Cl 2 TSV‐5/R cells were strongly reduced (approximately 1,000 fold). Their growth on plastic was also clearly slowed. Although the SV40 T antigen was still present in all the resistant cells they no longer had immunosensitivity to the SV40 specific graft rejection test. The caryotype of Cl 2 TSV‐5/R cells was comparable to that of the Cl 2 TSV‐5 cells.

📜 SIMILAR VOLUMES

Properties of an sv40-transformed africa

Properties of an sv40-transformed african green monkey kidney (bsc-1) cell line

✍ H. G. Suarez; M.-F. Bourali; R. Wicker; S. Estrade; R. Cassingena 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 French ⚖ 679 KB

## Abstract An SV40‐transformed BSC‐1 cell line (BSC‐SV Cl 1.1), that had been cloned twice under SV40 antiserum, sporadically produced small amounts of SV‐40 virus spontaneously. Most of the cells contained a defective virus genome, while some contained a non‐defective virus genome which was not r

Properties of transformed hamster cells

Properties of transformed hamster cells containing sv40 tumor antigen in the cytoplasm

✍ Linda S. Richardson; Janet S. Butel 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 French ⚖ 791 KB

## Abstract The properties of hamster cells containing SV40 tumor (T) antigen in the cytoplasm, rather than the nucleus, were determined. Eight cell lines were established from eight tumors induced by hamster embryo fibroblasts transformed __in vitro__ by PARA (2cT)‐adenovirus 7. Six cell lines con

New antigens in cells transformed by the

New antigens in cells transformed by the SV40 virus. II. Evidence of their cytoplasmic localization in a cell line (TSV5 CL2) of hamster fibroblasts transformed by SV40

✍ G. P. Tilz; Ch. De Vaux Saint Cyr; P. Grabar 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 French ⚖ 606 KB

A clone of hamster fibroblasts tramformed by SV40 virus (TSVKCL,) induced antibodies in hamsters against new antigenic constituents not present in the untransformed fibroblasts. The antibodies in the immune sera against histocompatibility antigens and those against nuclear T antigen were absorbed. T

Transformation of hamster cells in vivo

Transformation of hamster cells in vivo and in vitro by an adenovirus 4-SV40 hybrid

✍ John M. Easton; Alan S. Rabson; Richard A. Malmgren 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 French ⚖ 639 KB

## Abstract A preparation of adenovirus 4, containing infectious SV40 genetic material enclosed within adenovirus coats (Ad. 4‐SV40 hybrid), induced both transformation of newborn hamster kidney cells in vitro and tumors in neonatally inoculated hamsters. Injection of transformed cells into an irra

New antigens in cells transformed by the

New antigens in cells transformed by the SV40 virus. III. Presence of an SV40-coded antigen on the surface of different cell lines transformed by this virus

✍ A. Duthu-Herbet; M. Kress; Ch. De Vaux Saint Cyr 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 French ⚖ 669 KB

## Abstract The sera of hamsters carrying tumours induced by injection of TSV~5~Cl~2~ cells and the sera of animals immunized with these cells or with SV40 itself or its purified capsids have a slow cytotoxicity activity in the presence of fresh guinea pig complement on SV40‐transformed cell strain

The characterization of SV40-transformed

The characterization of SV40-transformed cell lines derived from mouse teratocarcinoma: Growth properties and differentiated characteristics

✍ W. Topp; J. D. Hall; D. Rifkin; A. J. Levine; R. Pollack 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 992 KB

## Abstract Mouse teratocarcinoma cells derived from embryoid bodies of 129SVsl mice were cultured in vitro to permit their differentiation. These cells were then infected with simiam virus 40 (SV40) and 31 cloned cell lines (SVTER) were derived from these cultures. All 31 SVTER cell lines containe