✦ LIBER ✦

Properties of a non-tumorigenic human cervical keratinocyte cell line

✍ Scribed by Margaret A. Stanley; Helena M. Browne; Mark Appleby; Anthony C. Minson

Publisher: John Wiley and Sons
Year: 1989
Tongue: French
Weight: 720 KB
Volume: 43
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910430422

No coin nor oath required. For personal study only.

✦ Synopsis

A human cervical keratinocyte cell line, W 12, has been initiated from a low-grade cervical lesion histologically diagnosed as CIN I. This cell line has, to date, undergone over 300 generations in vitro with an average doubling time of 30 hr: an aneuploid karyotype has developed with progressive in vitro growth. WI2 contains HPV16 DNA present at approximately 100 copies and the state of the viral DNA over a number of passages has been examined. The HPV16 DNA is stably maintained at high copy number over several passages and restriction enzyme analysis together with electrophoresis of uncleaved viral DNA indicate that it is present predominantly as the episomal molecule. W12 cells exhibit a typical keratinocyte morphology and, when transplanted into the flank of the nude mouse, form an epithelial lesion with the histological features of CIN 1/11.

📜 SIMILAR VOLUMES

Normal human chromosome 11 suppresses tu

Normal human chromosome 11 suppresses tumorigenicity of human cervical tumor cell line SiHa

✍ Minoru Koi; Hiroyuki Morita; Hideto Yamada; Hitoshi Satoh; J. Carl Barrett; Mits 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 984 KB

We examined the ability o f human chromosome 11 derived from normal fibroblast cells to suppress the tumorigenicity of SiHa cells, a human cervical tumor cell line. Using DNA transfection, the human chromosome was tagged with a selectable marker (the pSV2neo gene, which encodes resistance t o the an

Characterization of tumorigenic sub-line

Characterization of tumorigenic sub-lines from a poorly tumorigenic human colon-adenocarcinoma cell line

✍ Teresa López-Conejo; Nieves Olmo; Javier Turnay; Juana Navarro; Antonia Lizarbe 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 955 KB

The interaction of tumor cells with extracellular-matrix components is suspected to play an important role in tumorigenesis induction. The tumorigenicity of a poorly tumorigenic human colon-adenocarcinoma cell line (BCS-TC2) was induced by co-injection with Matrigel. A new cell sub-line, BCS-TC2. I,

Expression of cell surface glycoproteins

Expression of cell surface glycoproteins in human melanoma cell lines with different tumorigenic properties

✍ Odile Berthier-Vergnes; Jacques Portoukalian; Jean-François Doré 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 French ⚖ 784 KB

Human malignant melanoma cell lines characterized by either a high or a low ability to grow subcutaneously in athymic n-ude mice have been examined for their cell-surface glycoproteins. Striking differences were demonstrated between these 2 groups. Cells from lines of low tumorigenicity (LT group) d

Human chromosome II inhibits tumorigenic

Human chromosome II inhibits tumorigenicity of a murine squamous cell carcinoma cell line

✍ Jean C. Zenklusen; Mibuo Oshimura; J. Carl Barrett; Claudio J. Conti 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 538 KB

## Loss of heterozygosity (LOH ) of mouse chromosome 7 has been consistently demonstrated in chemically induced murine squamous cell carcinomas (SCCs). The region of this chromosome presenting LOH in the mouse tumors is syntenic to human chromosome segments I I p I 5 and I I q. To determine whethe

Different ligand responsiveness of human

Different ligand responsiveness of human retinoic-acid-receptor β-gene transcription in tumorigenic and non-tumorigenic cervical-carcinoma-derived cell lines is mediated through a large retinoic-acid-response domain

✍ Corinna Baust; Leslie Redpath; Elisabeth Schwarz 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 1020 KB

The retinoic-acid-receptor beta gene (RAR-beta) encodes a suspected tumor suppressor for several types of human carcinomas. RAR-beta transcription is induced by retinoic acid (RA) through retinoid receptors which bind as heterodimers of a RA-activated RA receptor (RAR) and a retinoid X receptor to t

IGF-2 autocrine stimulation in tumorigen

IGF-2 autocrine stimulation in tumorigenic clones of a human colon-carcinoma cell line

✍ Thomas Lamonerie; Christian Lavialle; Hedi Haddada; Olivier Brison 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 French ⚖ 716 KB

## Abstract Clonal analysis has shown that the SW613‐S human colon‐carcinoma cell line is heterogeneous; some cell clones display a high level of amplification of the c‐__myc__ gene and are tumorigenic in nude mice, whereas others have a small number of copies of this gene and are non‐tumorigenic.