𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression of cell surface glycoproteins in human melanoma cell lines with different tumorigenic properties

✍ Scribed by Odile Berthier-Vergnes; Jacques Portoukalian; Jean-François Doré

Publisher
John Wiley and Sons
Year
1985
Tongue
French
Weight
784 KB
Volume
36
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Human malignant melanoma cell lines characterized by either a high or a low ability to grow subcutaneously in athymic n-ude mice have been examined for their cell-surface glycoproteins. Striking differences were demonstrated between these 2 groups. Cells from lines of low tumorigenicity (LT group) displayed twice as much Vibrio cholerae neuraminidase and galactose oxidase accessible glycoproteins as cells from lines of high tumorigenicity (HT group) and each group of cell lines could be characterized by specific glycoprotein profiles. LT and HT group cells displayed similar amounts of periodate accessible glycoproteins, but sialoglycoprotein profiles were characteristic for each group of cell lines. Furthermore, whereas 87% of the sialic acid released by V. cholerae neuraminidase came from cell surface glycoproteins in HT group cells, only 53-55% of the released sialic acid came from surface glycoproteins in LT group cells. These results suggest that human melanoma cell lines exhibiting different tumorigenicity in nude mice can also be characterized by differences in composition and organization within the plasma membranes of their cell-surface sialoglycoproteins.

'To whom reprint requests should be addressed.

surface sialic acid residues susceptible to K cholerae

📜 SIMILAR VOLUMES

Lectin binding glycoproteins in human me
Lectin binding glycoproteins in human melanoma cell lines with high or low tumorigenicity
✍ Odile Berthier-Vergnes; Alain Réano; Jean-François Doré 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 French ⚖ 937 KB

Lectin binding glycoproteins of 5 human malignant melanoma cell lines (HMMCL), differing in their ability to grow subcutaneously in athymic nude mice, were compared by electrophoresis of total cellular proteins and subsequent incubation of SDS-poly-acrylamide gel with 'z51-labelled lectins. Despite

Intrinsic MDR-1 gene and P-glycoprotein
Intrinsic MDR-1 gene and P-glycoprotein expression in human melanoma cell lines
✍ Walter Berger; Leonilla Elbling; Michael Minai-Pour; Monika Vetterlein; Robert P 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 763 KB

Metastatic malignant melanoma is considered a chemotherapy-refractory malignancy. A few previous studies have delivered contradictory results regarding the presence and functionality of P-glycoprotein (P-gp), a transmembranous protein associated with the classical multidrug resistance (cMDR), in mal

Expression of tissue-type transglutamina
Expression of tissue-type transglutaminase correlates positively with metastatic properties of human melanoma cell lines
✍ Jan J. M. van Groningen; Steve L. Klink; Henri P. J. Bloemers; Guido W. M. Swart 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 700 KB

In this study the relationship between tissue-type transglutaminase (TGase2) activity and the propensity to metastasize was investigated in human melanoma cell lines with different metastatic behavior. TGase2 catalyzes an acyl-transfer reaction between peptide-bound glutamine residues and primary am

Detection in human melanoma cell lines o
Detection in human melanoma cell lines of particles with some properties in common with RNA tumour viruses
✍ P. G. Parsons; Patricia Goss; J. H. Pope 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 842 KB

## Abstract Six established lines of malignant melanoma cells produced particles having a buoyant density of 1.14 to 1.18 g/ml and containing RNA with a component of approximately 70S. Fractions containing these particles showed RNase‐sensitive DNA polymerase activity, and in simultaneous assays th

Expression levels of human P-glycoprotei
Expression levels of human P-glycoprotein in In Vitro cell lines: correlation between mRNA and protein levels for P-glycoprotein expressed in cells
✍ Yoshiyuki Shirasaka; Reiko Konishi; Nana Funami; Yuko Kadowaki; Yuri Nagai; Tosh 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 110 KB 👁 1 views

## Abstract The purpose of this study was to investigate the correlation between mRNA and protein levels for P‐glycoprotein (P‐gp) expressed in various cell lines to validate the estimation of P‐gp activity from its mRNA levels. P‐gp expression levels in various cell monolayers, normal, P‐gp‐induce

Integrin expression in human melanoma ce
Integrin expression in human melanoma cell lines: Heterogeneity of vitronectin receptor composition and function
✍ John F. Marshall; Stephen A. Nesbitt; Miep H. Helfrich; Michael A. Horton; Katar 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 957 KB

## Abstract Ten human melanoma cell lines were examined for integrin‐receptor expression using a panel of antibodies directed against different integrin subunits. Considerable heterogeneity was detected for levels of expression of 7 integrins, including the α~v~β~3~ vitronectin receptor where a cor