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Intrinsic MDR-1 gene and P-glycoprotein expression in human melanoma cell lines

✍ Scribed by Walter Berger; Leonilla Elbling; Michael Minai-Pour; Monika Vetterlein; Robert Pirker; Eva-Maria Kokoschka; Michael Micksche

Publisher: John Wiley and Sons
Year: 1994
Tongue: French
Weight: 763 KB
Volume: 59
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910590522

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✦ Synopsis

Metastatic malignant melanoma is considered a chemotherapy-refractory malignancy. A few previous studies have delivered contradictory results regarding the presence and functionality of P-glycoprotein (P-gp), a transmembranous protein associated with the classical multidrug resistance (cMDR), in malignant melanoma. Therefore we have investigated this issue on 33 cell lines established from primary and metastatic lesions of human malignant melanoma, comparing different cMDR detection methods. lmmunocytochemically 33% of the cell lines stained positive for P-gp. The data correlated with those of a P-gp-radioimmunometric (antibody-binding) assay. When RT-PCR was used for MDR-I mRNA determination, 76% of the melanoma cell lines scored positive. Slot-blot 5To whom correspondence and reprint requests should be sent, at

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