Proliferation of chick primordial germ c
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Il-Kuk Chang; Atsushi Tajima; Tateki Chikamune; Tadao Ohno
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Article
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1995
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Elsevier Science
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English
β 471 KB
Fluorescent reagentβlabelled PGCs isolated from the blood of 2βdayβold chick embryos were cultured on stroma cells derived from 5βdayβold germinal ridge in Medium 199 supplemented with 10% FBS, human IGFβ1, bovine FGFβb, and murine LIF. In 7 experiments, the number of MCs increased by an average of