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Proliferation of alkaline phosphatase-positive cells from cultured primordial germ cells in rats

✍ Scribed by T. Mitani; N. Takahashi; E. Kawase; K. Hashimoto


Book ID
119498282
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
65 KB
Volume
41
Category
Article
ISSN
0093-691X

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Fluorescent reagent‐labelled PGCs isolated from the blood of 2‐day‐old chick embryos were cultured on stroma cells derived from 5‐day‐old germinal ridge in Medium 199 supplemented with 10% FBS, human IGF‐1, bovine FGF‐b, and murine LIF. In 7 experiments, the number of MCs increased by an average of