Proliferating cell nuclear antigen (PCNA) is expressed in activated rat skeletal muscle satellite cells

✍ Scribed by Sally E. Johnson; Ronald E. Allen

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 577 KB
• Volume: 154
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041540106

✦ Synopsis

Skeletal muscle satellite cells from uninjured muscle of adult animals are generally found to be in a quiescent state, and when cultured, they remain quiescent in vitro for a period of time which i5 directly related to the age of the donor animal. A technique for studying the activation of satellite cells in primary cultures has been developed and employs proliferating cell nuclear antigen (PCNA) as a marker for entrance into the S phase of the cell cycle. PCNA is a protein involved in DNA replication and is maximally expressed in S phase of the cell cycle. We monitored PCNA expression in satellite cells isolated from young (3 week) arid adult (9 month) rats, and our results indicate that satellite cells begin to accumulate PCNA prior to changes in cell number in 130th age groups. Using El-ISA techniques, we demonstrated that addition of an extract of crushed muscle (CME) activated satel-Iitc cells and significantly reduced the length of the lag pha5e in cells from both age groups. Addition of bFCF shortened the lag phase o i PCNA synthesis in satellite cells from 3-week-old rats but had no effect on the kinetics of PCNA expression in cells from 9-month-old rats. Based on our experiments, PCNA expression can be used as a marker to follow the entry of satellite cells into thc cell cycle in primary nlass cultures.

'G, 1993 Wilcy-Lis, Irrc.