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A cryptopromoter is activated in the proliferating cell nuclear antigen gene of growth arrested cells

✍ Scribed by Christian Sell; Chung-Der Chang; Janice Koniecki; Hui-Min Chen; Renato Baserga

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
846 KB
Volume
152
Category
Article
ISSN
0021-9541

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✦ Synopsis

Massachusetts 02 125

We have examined the regulation of the proliferating cell nuclear antigen gene (PCNA) in a hamster fibroblast cell line (tk-tsl3) which is temperature sensitive for growth. These tk-tsl3 cells, at the restrictive temperature, are growth arrested in the G, phase of the cell cycle. The cells were stably transfected with a full length human PCNA gene, and the resulting cell lines (K525 cells) were analyzed. We find that, in growth arrested K525 cells, a cryptopromoter is activated in the transfected human PCNA gene. The cryptopromoter resides in intron 4 which is necessary for proper regulation of the PCNA gene. Removal of this intron leads to increased expression of PCNA in cells which have entered the Go state. An Alu sequence residing in intron 4 is implicated as the promoter element which is active during growth arrest, D 1992 WiIPy-iiss, \tic.

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