Prognostic values of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in bladder cancer

✍ Scribed by Hiro-omi Kanayama; Kin-ya Yokota; Yasushi Kurokawa; Yoshihide Murakami; Masaaki Nishitani; Susumu Kagawa

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 142 KB
• Volume: 82
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19980401)82:7<1359::aid-cncr20>3.0.co;2-4

✦ Synopsis

Background:

Matrix metalloproteinase-2 (mmp-2), which degrades the extracellular matrix or the basement membrane, has an essential role in tumor invasion and metastasis. to evaluate the roles of mmp-2, its inhibitor (tissue inhibitor of metalloproteinase-2 [timp-2]), and its activator (membrane-type matrix metalloproteinase-1 [mt1-mmp]) in tumor invasion or as a prognostic factor in patients with bladder carcinoma, the authors investigated the expression of mmp-2, timp-2, and mt1-mmp in patients with bladder carcinoma.

Methods:

Tissues obtained from 41 patients with bladder carcinoma were used for analysis. expression of mmp-2, timp-2, mt1-mmp, and glyceraldehyde-3-phosphate dehydrogenase was examined by reverse transcriptase-polymerase chain reaction analysis. correlations between the levels of mmp-2, timp-2, and mt1-mmp expression and histologic findings or patient outcome were evaluated.

Results:

Expression of mmp-2 and timp-2 was significantly higher in muscle invasive pt2< or = bladder tumors than in pt1a tumors (mmp-2: p < 0.0005; timp-2: p < 0.005). moreover, high levels of mmp-2 and timp-2, as well as mt1-mmp expression, all were strongly associated with decreased survival (mmp-2: p < 0.0001; timp-2: p < 0.0001; and mt1-mmp: p < 0.005). even within the radically cystectomized muscle invasive pt2< or = tumor group, patients with a high expression of any of these three genes had a worse prognosis than those with low expression (mmp-2: p < 0.05; timp-2: p < 0.05; and mt1-mmp: p = 0.0641).

Conclusions:

Mmp-2 and timp-2 are believed to contribute to the invasive properties of bladder carcinoma. the authors report that expression of mmp-2, timp-2, and mt1-mmp are useful prognostic indicators in patients with bladder carcinoma and may be helpful in designing treatment protocols.