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Production, purification, and functional analysis of recombinant human and mouse 17β-hydroxysteroid dehydrogenase type 7

✍ Scribed by Svea Törn; Pasi Nokelainen; Riitta Kurkela; Anitta Pulkka; Marta Menjivar; Sikha Ghosh; Miguel Coca-Prados; Hellevi Peltoketo; Veli Isomaa; Pirkko Vihko

Book ID
117053837
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
375 KB
Volume
305
Category
Article
ISSN
0006-291X

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Expression, Purification, and Characteri
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✍ Yi-Hsun Chang; Yuan-Liang Wang; Jain-Yu Lin; Lea-Yea Chuang; Chi-Ching Hwang 📂 Article 📅 2009 🏛 Humana Press Inc 🌐 English ⚖ 311 KB

Human 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reaction of estrone with NADPH to form estradiol and NADP(+), thereby regulating the biological activity of sex steroid hormones in a variety of tissues. Here, we present an efficient method for expressing and purifying hum