Expression, Purification, and Characterization of a Human Recombinant 17β-Hydroxysteroid Dehydrogenase Type 1 inEscherichia coli

✍ Scribed by Yi-Hsun Chang; Yuan-Liang Wang; Jain-Yu Lin; Lea-Yea Chuang; Chi-Ching Hwang

• Book ID: 107581766
• Publisher: Humana Press Inc
• Year: 2009
• Tongue: English
• Weight: 311 KB
• Volume: 44
• Category: Article
• ISSN: 1073-6085
• DOI: 10.1007/s12033-009-9221-5

✦ Synopsis

Human 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reaction of estrone with NADPH to form estradiol and NADP(+), thereby regulating the biological activity of sex steroid hormones in a variety of tissues. Here, we present an efficient method for expressing and purifying human 17beta-HSD1 from Escherichia coli. The expression vector pET28a/17beta-HSD1 was constructed and transformed into Escherichia coli BL21(DE3) cells. We found that the active enzyme can be obtained by inducing 17beta-HSD1 expression at 0.25 mM IPTG, 13 degrees C for overnight. The protein is purified by single step Ni-NTA affinity chromatography and yields 2.8 mg/L of culture. The kinetic study shows V/E ( t ) of (1.21 +/- 0.05) x 10(-2)/s and K (estradiol) of 0.8 microM in the oxidation of estradiol with NADP(+) as cofactor at pH 9.3. The new bacterial expression system for recombinant 17beta-HSD1 is useful for the easy purification of large amounts and will facilitate the functional study of this enzyme.