𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression, Purification, and Characterization of a Human Recombinant 17β-Hydroxysteroid Dehydrogenase Type 1 inEscherichia coli

✍ Scribed by Yi-Hsun Chang; Yuan-Liang Wang; Jain-Yu Lin; Lea-Yea Chuang; Chi-Ching Hwang


Book ID
107581765
Publisher
Humana Press Inc
Year
2009
Tongue
English
Weight
311 KB
Volume
44
Category
Article
ISSN
1073-6085

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Expression, Purification, and Characteri
✍ Yi-Hsun Chang; Yuan-Liang Wang; Jain-Yu Lin; Lea-Yea Chuang; Chi-Ching Hwang 📂 Article 📅 2009 🏛 Humana Press Inc 🌐 English ⚖ 311 KB

Human 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reaction of estrone with NADPH to form estradiol and NADP(+), thereby regulating the biological activity of sex steroid hormones in a variety of tissues. Here, we present an efficient method for expressing and purifying hum

Characterization of estrogen-dependent g
✍ Minna M. Miettinen; Matti H. Poutanen; Reijo K. Vihko 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 559 KB

I7f3-hydroxysteroid dehydrogenase (I 7HSD) type I converts the weakly active estrogen, estrone, into highly active estradiol. In addition to being essential for gonadal estradiol biosynthesis, the enzyme is also expressed in a significant proportion of breast tumors. In order to study the role of th