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Cloning, High Expression and Purification of Recombinant Human Intereferon-β-1b inEscherichia coli

✍ Scribed by Dasari Venkata Krishna Rao; Chatadi Tulasi Ramu; Joginapally Venkateswara Rao; Mangamoori Lakshmi Narasu; Adibhatla Kali Satya Bhujanga Rao


Book ID
107565008
Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
255 KB
Volume
158
Category
Article
ISSN
0273-2289

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Human 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reaction of estrone with NADPH to form estradiol and NADP(+), thereby regulating the biological activity of sex steroid hormones in a variety of tissues. Here, we present an efficient method for expressing and purifying hum