Three elicitor fractions including spent culture medium, hydrolyzed mycelial pellet, and hydrolyzed mycelial biomass supematafif'were tested for their ability to induce sesquiterpene formation in freely suspended and immobilized cells. Immobilization enhanced the effectiveness of the hydrolysate eli
Production of solavetivone by immobilized cells ofHyoscyamus muticus
โ Scribed by S. V. Ramakrishna; G. Ramakrishna Reddy; Wayne R. Curtis; Arthur E. Humphrey
- Book ID
- 104633016
- Publisher
- Springer Netherlands
- Year
- 1993
- Tongue
- English
- Weight
- 344 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0141-5492
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โฆ Synopsis
The secretion of solavetivone, a phytoalexin, by the cells of Hyoscyamus muticus in response to fungal elicitation has been enhanced by gel entrapment in calcium alginate. The immobilized cells produced 53% higher product and exhibited sustained biosynthetic activity in repeated batch cycles when compared with suspended cells. Providing a non-elicited media exchange and a sufficient interval of time between repeated infection (elicitation) gave greater productivity. Apparently the cells need a period to recover from the infection.
๐ SIMILAR VOLUMES
Columnar reactors containing immobilized cells of Zymomonas mobilis were utilized for the continuous production of ethanol from glucose. Two different immobilization strategies were investigated. In one case, cells were entrapped in borosilicate glass fiber pads, while in the other, cells were immo
Cells of Saccharomyces cerevisiae were immobilized in K-Carrageenan. Addition of sodium sulfite to the fermentation medium up to four percent led to glycerol yields of 25 to 27 g/l at temperatures below 31 ~ These results demonstrate that it is possible to direct the metabolism of immobilized cells