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Stimulation of solavetivone synthesis in free and immobilized cells ofHyoscyamus muticusbyRhizoctonia solanifungal components

✍ Scribed by S. V. Ramakrishna; G. Ramakrishna Reddy; Wayne R. Curtis; Arthur E. Humphrey

Book ID
104633017
Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
258 KB
Volume
15
Category
Article
ISSN
0141-5492

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✦ Synopsis

Three elicitor fractions including spent culture medium, hydrolyzed mycelial pellet, and hydrolyzed mycelial biomass supematafif'were tested for their ability to induce sesquiterpene formation in freely suspended and immobilized cells. Immobilization enhanced the effectiveness of the hydrolysate elicitors, but not that derived from spent culture medium. When effectiveness of the elicitors is compared based on product yield, spent culture medium is shown to be far more effective than hydrolysates as an elicitor.

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Sodium orthovanadate stimulation of DNA synthesis in nakano mouse lens epithelial cells in serum-free medium
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## Abstract Quiescent cultured Nakano mouse lens cells incubated for 40 hours with sodium orthovanadate incorporated ^3^H‐thymidine at an accelerated rate; the greatest response occurred at 20 ΞΌM vanadate, whereas by 2 ΞΌM an incorporation rate equivalent to unstimulated cells was noted. Microscopic