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Production of recombinant proteins in clonal root cultures using episomal expression vectors

✍ Scribed by Marina Skarjinskaia; Jason Karl; Adriana Araujo; Karen Ruby; Shailaja Rabindran; Stephen J. Streatfield; Vidadi Yusibov

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
304 KB
Volume
100
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

We have developed a fully contained system for expressing recombinant proteins that is based on clonal root cultures and episomal expression vectors. Clonal root lines expressing green fluorescent protein (GFP) or human growth hormone were generated from Nicotiana benthamiana leaves infected with the tobacco mosaic virus‐based vector 30B after exposure to Agrobacterium rhizogenes. These lines accumulated GFP at over 50 mg per kg fresh tissue, a level that is comparable with other plant production systems in early stage development. Accumulation of both hGH and GFP in the clonal root lines was sustained over a 3‐year period, and in the absence of antibiotic selection. This technology shows promise for commercial production of vaccine antigens and therapeutic proteins in contained facilities. Biotechnol. Bioeng. 2008;100: 814–819. Β© 2008 Wiley Periodicals, Inc.

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