Production of recombinant human midkine in yeast, Pichia pastoris
β Scribed by Akira Murasugi; Yumiko Tohma-Aiba; Yukio Asami
- Book ID
- 114398921
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 647 KB
- Volume
- 90
- Category
- Article
- ISSN
- 1389-1723
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## Abstract __Microsomal cytochrome P450 monooxygenases of groups 1β3 are mainly expressed in the liver and play a crucial role in phase 1 reactions of xenobiotic metabolism. The cDNAs encoding human CYP2D6 and human NADPHβP450 oxidoreductase (CPR) were transformed into the methylotrophic yeast__ P
The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels. Using this system, recent advances have been made in the quality of recombinant proteins in fermenter culture and in the quality of