Production of native recombinant human midkine in the yeast, Pichia pastoris
β Scribed by Akira Murasugi; Yumiko Tohma-Aiba
- Book ID
- 117702594
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 177 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1046-5928
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## Abstract __Microsomal cytochrome P450 monooxygenases of groups 1β3 are mainly expressed in the liver and play a crucial role in phase 1 reactions of xenobiotic metabolism. The cDNAs encoding human CYP2D6 and human NADPHβP450 oxidoreductase (CPR) were transformed into the methylotrophic yeast__ P
The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels. Using this system, recent advances have been made in the quality of recombinant proteins in fermenter culture and in the quality of