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Production of murine monoclonal antibodies against human thyroglobulin using an in vitro immunization procedure in serum-free medium

โœ Scribed by Ferry A. Ossendorp; Mark De Boer; Bert J.M. Al; Jo Hilgers; Peter F. Bruning; Joseph M. Tager

Book ID
118900469
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
620 KB
Volume
91
Category
Article
ISSN
0022-1759

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Stimulation of monoclonal antibody produ
Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium
โœ S. Sato; H. Murakami; T. Sugahara; T. Ikegami; K. Yamada; H. Omura; S. Hashizume ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 325 KB

Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml