𝔖 Bobbio Scriptorium
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Production of monoclonal antibodies in serum free medium

✍ Scribed by Tong H. Chang; Zenon Steplewski; Hilary Koprowski


Book ID
118898066
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
362 KB
Volume
39
Category
Article
ISSN
0022-1759

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Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml