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Production of L-aminoacylase by fermentation of Pseudomonas sp. BA2

✍ Scribed by Antonio Bódalo Santoyo; Josefa Bastida Rodríguez; José Luis Gómez Carrasco; Elisa Gómez Gómez; María Luisa Asanza Teruel; Isabel Alcaraz Rojo

Publisher
Wiley (John Wiley & Sons)
Year
1995
Tongue
English
Weight
523 KB
Volume
64
Category
Article
ISSN
0268-2575

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✦ Synopsis

The growth and enzymatic production of Pseudomonas sp. BA2, a new L-aminoacylase-producing microorganism, were studied in a bench-top fermenter. Multiple fermentations were carried out in order to determine the optimal pH and temperature values. The influence of the substrate concentration on both growth and L-aminoacylase activity was also investigated. The maximum growth rate and the greatest yield of enzyme were obtained when the fermentation was carried out at pH 7.5, 25°C and DOT > 50%. N-Acetyl-DLalanine, at a concentration of 20 g dm-3, was used as the sole carbon and nitrogen source. The fermentation process provided a maximum biomass concentration of 3.36 g dry weight dm-3. The highest L-aminoacylase production (11429 U g-' dry weight) was obtained after 39 h of cultivation. The results were a significant improvement over those previously reported.

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