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Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors

✍ Scribed by Fairolniza Mohd. Shariff; Thean Chor Leow; A. D. Mukred; Abu Bakar Salleh; Mahiran Basri; Raja Noor Zaliha Raja Abd. Rahman

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
281 KB
Volume
47
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 Β°C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca^2+^ and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 Β°C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa. (Β© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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## Abstract Lipase production by the mutant strain __Rhizopus__ sp. BTNT‐2 was optimized in submerged fermentation. Different chemical and physical parameters such as carbon sources, nitrogen sources, oils, inoculum level, pH, incubation time, incubation temperature and aeration have been extensive