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Production of human antibody repertoires in transgenic mice

✍ Scribed by Marianne Brüggemann; Michael J Taussig

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
485 KB
Volume
8
Category
Article
ISSN
0958-1669

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✦ Synopsis

Transgenic mice have been created that carry human immunoglobulin heavy and light chain genes in germline configuration and that have the corresponding endogenous genes silenced. The transgenes are either minigene constructs or large, almost authentic, transloci on yeast artificial chromosomes and undergo B-cell-specific DNA rearrangement and hypermutation in the mouse lymphoid tissue. Monoclonal antibodies with good affinities for human antigens have been obtained after immunisation. These mice may be a future source of human antibodies for therapy.

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