Reduction in alkaline phosphatase activity was observed when HeLa S3 cells were grown in Puck's medium containing high concentrations of human serum. This effect was not seen with the enzyme of Chang liver 8A cells. The induction of increased alkaline phosphatase in HeLa S3 by prednisolone or by osm
Production of fetal-like alkaline phosphatase by HeLa cells
β Scribed by Norton A. Elson; Rody P. Cox
- Publisher
- Springer
- Year
- 1969
- Tongue
- English
- Weight
- 791 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0006-2928
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The techniques for measurement of biosynthetic rates and intracellular transit times of an integral membrane protein isoenzyme have now been validated. Thus, induction of placental alkaline phosphatase in cultured HeLa cells by prednisolone and by butyrate is shown to result in its increased biosynt
## Abstract Nonβlethal concentrations of bromodeoxyuridine induce a 2β to 5βfold increase in the specific activity of alkaline phosphatase in HeLa subclone, S3G. Experiments employing 10βhour pulses of BRdU showed that 48 hours were required before induction commenced, and that maximal induction wa
## Abstract Cultures of HeLa cells, strains S3G (HeLa~65~) and S3K (HeLa~71~) were grown in plastic dishes until firmly attached and were then treated with sonic dispersions of Rosenthal's phospholipase inhibitor. A rapid increase in alkaline phosphatase activity occurred following this treatment i