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Production of anti-rubratoxin antibody and its use in a radioimmunoassay for rubratoxin B

✍ Scribed by Robert M. Davis; Stanley S. Stone

Publisher
Springer Netherlands
Year
1979
Tongue
English
Weight
372 KB
Volume
67
Category
Article
ISSN
0301-486X

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✦ Synopsis

Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HC1 (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratoxin antibody was produced. A radioimmunoassay for rubratoxin B was developed which could detect 0.1 #g to 10 #g of toxin using 0.21 #g of [-t ~C] rubratoxin (0.47 Ci/mole, 2.0 x 109 dpm/#g) and 0.125 ml of anti-rubratoxin antibody.

Rubratoxicosis was first described in 1957 by Burnside et al. (3) after finding that corn infected with Penicillium rubrum Stoll caused death in swine. Wilson and Wilson

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