Production of actinorhodin by Streptomyces coelicolor A3(2) grown in chemostat culture

✍ Scribed by Karel Melzoch; M. Joost Teixeira de Mattos; Oense M. Neijssel

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 195 KB
• Volume: 54
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19970620)54:6<577::aid-bit8>3.0.co;2-g

✦ Synopsis

Streptomyces coelicolor was grown in variously culture (i.e., growth rate of the organism), the culture limited chemostat cultures and the specific rate of extra-pH value, and other factors. In many cases, a physiologicellular actinorhodin production (q actinorhodin ) was meacal explanation for the production of a particular comsured. The highest q actinorhodin values were observed in glupound can be given (Neijssel et al., 1993;Neijssel and cose-or ammonia-limited cultures, whereas almost no Tempest, 1975).

actinorhodin was produced in sulfate-, phosphate-, pot-Whereas overflow metabolism concerns primary meassium-, or magnesium-limited cultures. The effect of the dilution rate on actinorhodin production was studied in tabolites, the synthesis and overproduction of another glucose-limited cultures. It was found that q actinorhodin was class of compounds, the secondary metabolites, has highest at D ϭ 0.06 h Ϫ1 , which was well below the maximal been studied mostly via genetic methods. The physio-D value tested (0.14 h Ϫ1 ). This explains why, in batch logical significance of the production of these comcultures, actinorhodin production starts at the onset of pounds is still unclear (Piepersberg, 1993;Vining, 1990), the stationary phase. It was also found that the use of nitrilotriacetate instead of citrate as a chelating agent had which makes the development of a production strategy a negative effect on actinorhodin production.