Oxygen transfer and uptake in Streptomyces coelicolor A3(2) culture in a batch bioreactor

✍ Scribed by Kutlu Ozergin-Ulgen; Ferda Mavituna

• Publisher: Wiley (John Wiley & Sons)
• Year: 1998
• Tongue: English
• Weight: 248 KB
• Volume: 73
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/(sici)1097-4660(1998110)73:3<243::aid-jctb957>3.0.co;2-5

✦ Synopsis

This paper provides quantitative information on oxygen transfer as well as the kinetic and metabolic parameters related to oxygen uptake in Streptomyces coelicolor A3(2) cultured in a 20 dm3 computer controlled bioreactor using both deÐned and complex media. It is evident from the literature that production of antibiotics is strongly a †ected by the dissolved oxygen concentration. Many processes of antibiotic fermentations have been developed to the point at which the microbial oxygen demand exceeds the oxygen transfer capability of the existing fermentation facilities. As a consequence, the oxygen transfer rate has become the rate limiting factor in such processes. It is necessary to know the oxygen kinetic and metabolic parameters of an aerobic fermentation for a successful scale-up and operational control of the process. In the literature, information concerning the oxygen uptake kinetics of the Streptomyces cultures is scarce despite their industrial importance. This paper, therefore, provides useful quantitative information on oxygen transfer and uptake rates in S. coelicolor cultures. In the deÐned medium, the total oxygen uptake rates were in the range of 5È6 mmol dm~3 h~1 throughout the active growth phase, the maximum O 2 speciÐc oxygen uptake rate was 7É44 mmol g cell~1 h~1, the speciÐc oxygen O 2 maintenance demand was 1É88 mmol g cell~1 h~1, and the values were in O 2 k L a the range of 40È100 h~1. In the complex medium, however, the values varied k L a in the range of 18È70 h~1.