Production of 4,5-dihydroxyphthalate by Pseudomonas testosteroni immobilized in alginate gel beads

✍ Scribed by Akihiko Sakurai; Mikio Sakakibara

• Book ID: 104420300
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 127 KB
• Volume: 3
• Category: Article
• ISSN: 1369-703X
• DOI: 10.1016/s1369-703x(99)00008-x

✦ Synopsis

4,5-Dihydroxyphthalate (DHP) production was carried out using Pseudomonas testosteroni immobilized in alginate gel beads. Strontium chloride was more suitable for a gelation agent with regard to DHP production than calcium chloride or barium chloride. The optimal gelation time was about 0.5 h, and the DHP production rate decreased with an increase in gelation time. The optimal pH for DHP production using the immobilized cells was shifted about 1.0 to the alkali side compared with that for the suspended cells. A repeated batch operation using the immobilized cells was carried out. It was effective for ef®cient DHP production, and a high production rate was maintained to the ®fth cycle.